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Catalog number: SBB-KP0038, 100 x 50 μL reactions
Catalog number: SBB-KP0038 This kit is designed to test for specific activity of 20S proteasome. The kit provides purified 20S proteasome and is designed to test for Chymotrypsin-like activity (Suc-LLVY-AMC), Caspase-like activity of the proteasome subunits ß1/PSMB6 (LLE-AMC), and Chymotrypsin-like activity of ß5/PSMB5 (WLA-AMC). [1][2][3][4]
$425.00
Catalog number: SBB-KP0038, 100 x 50 μL reactions
This kit is designed to test for specific activity of 20S proteasome. The kit provides purified 20S proteasome and is designed to test for Chymotrypsin-like activity (Suc-LLVY-AMC), Caspase-like activity of the proteasome subunits ß1/PSMB6 (LLE-AMC), and Chymotrypsin-like activity of ß5/PSMB5 (WLA-AMC).
Quantity
Total
$425.00
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| Quantity: | 100 x 50 μL reactions |
|---|---|
| Molecular Weight: | — |
| Purity: | > 95% |
| Readout: | Endpoint / Kinetic |
| Label or Dye: | 7-Amino-4-methylcoumarin |
| Detection Method: | Fluorescence |
| Substrate Properties: | Protein-Based Substrate |
| Excitation/Emission (nm): | 345/445 |
| Storage Buffer: | — |
| Storage | -80C, Avoid multiple freeze / thaw cycles. It is recommended to make aliquots of each reaction component upon first time use. |
Certificate of Analysis
Open Certificate in New TabCitations and References
1) Smith DM, Chang SC, Park S, Finley D, Cheng Y, Goldberg AL (Sep 2007). “Docking of the proteasomal ATPases’ carboxyl termini in the 20S proteasome’s alpha ring opens the gate for substrate entry”. Molecular Cell. 27 (5): 731–44. doi:10.1016/j.molcel.2007.06.033. PMC 2083707Freely accessible. PMID 17803938. 2) Wilk S, Orlowski M (Mar 1983). “Evidence that pituitary cation-sensitive neutral endopeptidase is a multicatalytic protease complex”. Journal of Neurochemistry. 40 (3): 842–9. doi:10.1111/j.1471-4159.1983.tb08056.x. PMID 6338156. 3) Kisselev, Alexei F., and Alfred L. Goldberg. “Monitoring activity and inhibition of 26S proteasomes with fluorogenic peptide substrates.” Methods in enzymology 398 (2005): 364-378. 4) Ettari, Roberta, et al. “Immunoproteasome-selective inhibitors: a promising strategy to treat hematologic malignancies, autoimmune and inflammatory diseases.” Current medicinal chemistry 23.12 (2016): 1217-1238.
