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Catalog number: SBB-DE0127, 50 g
The severe acute respiratory syndrome coronavirus papain-like protease (SARS-CoV-2 PLpro) is involved in the processing of the viral polyprotein. Proteolytic processing of the coronavirus replicase poly-protein is essential for generating a functional virus replication complex. PLpro possesses both deubiquitinating or deISGylating activity and can process Lys- 48 and Lys-63 linked polyubiquitin chains (free chains or from cellular substrates). It works in concert together with nsp4 in the assembly of virally-induced cytoplasmic double-membrane vesicles necessary for viral replication. It strongly antagonizes the innate immune induction of type I interferon by blocking the phosphorylation, dimerization and therefore the nuclear translocation of host IRF3. In addition, it prevents also host NF-kappa-B signaling. SARS Cov-2 PLpro is very active hydrolyzing both ISG15- Rhodamine110 (SBB-PS0002) or di-ubiquitin/ tetra-ubiquitin substrates (Lys48 or Lys63 linked), but is very inefficient when processing mono-Ub conjugates e.g. Ub-AMC or synthetic peptide substrates e.g. RLRGG-AMC. This SARS Coronavirus recombinant PLpro is N-terminally His10-tagged and expressed in E.coli.
$189.00
Catalog number: SBB-DE0127, 50 g
The severe acute respiratory syndrome coronavirus papain-like protease (SARS-CoV-2 PLpro) is involved in the processing of the viral polyprotein. Proteolytic processing of the coronavirus replicase poly-protein is essential for generating a functional virus replication complex. PLpro possesses both deubiquitinating or deISGylating activity and can process Lys- 48 and Lys-63 linked polyubiquitin chains (free chains or from cellular substrates).
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$189.00
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| Quantity: | 50 µg |
|---|---|
| Molecular Weight: | 37.6 kDa |
| Purity: | >95% by SDS-PAGE |
| Storage Buffer: | 50 mM HEPES pH 7.5, 100 mM NaCl, 1 mM TCEP |
| Storage | Store at −80°C after product arrival. Avoid multiple freeze/thaws. |
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Open Certificate in New TabCitations and References
1) Fran Robson et al. “Coronavirus RNA Proofreading: Molecular Basis and Therapeutic Targeting”. Molecular Cell, VOLUME 79, ISSUE 5, P710-727, SEPTEMBER 03, 2020, https://doi.org/10.1016/j.molcel.2020.07.027 2) Gordon, D.E., Jang, G.M., Bouhaddou, M. et al. A SARS-CoV-2 protein interaction map reveals targets for drug repurposing. Nature 583, 459–468 (2020). https://doi. org/10.1038/s41586-020-2286-9 3) Rota, P. A. “Characterization Of A Novel Coronavirus Associated With Severe Acute Respiratory Syndrome”. Science, vol 300, no. 5624, 2003, pp. 1394-1399. American Association For The Advancement Of Science (AAAS), doi:10.1126/science.1085952. 4) Jozlyn R. Clasman, Renata K. Everett, Karthik Srinivasan, Andrew D. Mesecar, Decoupling deISGylating and deubiquitinating activities of the MERS virus papain-like protease, Antiviral Research, Volume 174, 2020, 104661, ISSN 0166-3542, https://doi.org/10.1016/j. antiviral.2019.104661.
