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Catalog number: SBB-PS0002, 50 μg
This product consists of a full-length, mature ISG15 polypeptide (amino acids 2-157) recombinantly expressed in E.coli, conjugated on its c-terminus to a quenched Rhodamine110 dye. Hydrolysis of the conjugate results in fluorescence observable by excitation at 485nm and emission at 535nm, which substantially reduces the risk of autofluorescence of compounds in screenings (Hassapien et al., 2007). Typical working range is 50-500nM.
$295.00
Catalog number: SBB-PS0002, 50 μg
This product consists of a full-length, mature ISG15 polypeptide (amino acids 2-157) recombinantly expressed in E.coli, conjugated on its c-terminus to a quenched Rhodamine110 dye. Hydrolysis of the conjugate results in fluorescence observable by excitation at 485nm and emission at 535nm, which substantially reduces the risk of autofluorescence of compounds in screenings (Hassapien et al., 2007). Typical working range is 50-500nM.
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$295.00
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| Quantity: | 50 μg |
|---|---|
| Molecular Weight: | 17.6kDa |
| Purity: | >97% by LCMS |
| Readout: | Endpoint / Kinetic |
| Label or Dye: | Rhodamine 110 |
| Detection Method: | Fluorescence |
| Substrate Properties: | Protein-Based Substrate |
| Excitation/Emission (nm): | 485/535 |
| Storage Buffer: | 50mM MES pH 6.0, 100mM NaCl, 10% Glycerol |
| Storage | — |
Certificate of Analysis
Open Certificate in New TabCitations and References
1) Basters, et al. (2012) High yield expression of catalytically active USP18 (UBP43) using a Trigger Factor fusion system. BMC Biotechnology 12:56.
