Catalog number: SBB-PS0002, 50 μgThis product consists of a full-length, mature ISG15 polypeptide (amino acids 2-157) recombinantly expressed in E.coli, conjugated on its c-terminus to a quenched Rhodamine110 dye. Hydrolysis of the conjugate results in fluorescence observable by excitation at 485nm and emission at 535nm, which substantially reduces the risk of autofluorescence of compounds in screenings (Hassapien et al., 2007). Typical working range is 50-500nM.