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Catalog number: SBB-KF0056, 400 wells
The kit uses ubiquitin labeled with either Europium-Cryptate or Cyanine5 as FRET pair donor and acceptor fluorophores respectively, completely eliminating the need for antibody based detection setups. Enzymatic incorporation of the labeled ubiquitins into chains conjugated onto NEDD4 leads to an increase in fluorescence emission at 665 nm (Emacceptor) and decrease at emission wavelength 620 nm (Emdonor).
$850.00
Catalog number: SBB-KF0056, 400 wells
The kit uses ubiquitin labeled with either Europium-Cryptate or Cyanine5 as FRET pair donor and acceptor fluorophores respectively, completely eliminating the need for antibody based detection setups. Enzymatic incorporation of the labeled ubiquitins into chains conjugated onto NEDD4 leads to an increase in fluorescence emission at 665 nm (Emacceptor) and decrease at emission wavelength 620 nm (Emdonor).
Quantity
Total
$850.00
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| Quantity: | 400 wells (20μl / well) |
|---|---|
| Readout: | Endpoint/Kinetic |
| Label or Dye: | Europium Cryptate & Cy5 |
| Detection Method: | TR-FRET |
| Substrate Properties: | Protein-Based Substrate. Typical experimental concentration is 1x. |
| Excitation/Emission (nm): | 304/620 & 304/665 |
| Storage | Store at −80°C after product arrival. Avoid multiple freeze / thaws. It is recommended to make multiple aliquots after the first thaw. |
Certificate of Analysis
Open Certificate in New TabCitations and References
1) Magennis, S. W., Parsons, S., Pikramenou, Z., Corval, A., & Woollins, J. D. (1999). Imidodiphosphinate ligands as antenna units in luminescent lanthanide complexes.Chemical communications, (1), 61-62. 2) Zheng, N., & Shabek, N. (2017). Ubiquitin Ligases: Structure, Function, and Regulation.Annual Review of Biochemistry, (0).
