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Catalog number: SBB-PS0044, 50 μg
NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8) is a ubiquitin like protein that plays an important role in regulating development and the cell cycle. NEDD8 is conjugated to a target protein via a signaling cascade similar to ubiquitin: a NEDD8 specific E1 activating enzyme (APPBP1/UBA3) adenylates the c-terimnus of NEDD8 which is than subsequently passed to an E2 conjugating enzyme (UBE2M or UBE2F) that facilitates covalent attachment of NEDD8 to a cullin subunit of an SCF E3 ubiquitin ligase. NEDD8-Rh 110 can be used as a substrate for enzymes exhibiting deNEDDylating activity, e.g UCH-L3, UCH-L1, COP9 Signalosome, and NEDP1. This product consists of a full-length, mature NEDD8 polypeptide (amino acids 1-76) recombinantly expressed in E.coli, conjugated on its c-terminus to a quenched AMC110 dye. Once hydrolyzed the free AMC provides excellent utility for real time assessment of enzyme activity at excitation (345 nm) and emission (445 nm). Typical working range is 50-500nM.
$245.00
Catalog number: SBB-PS0044, 50 μg
This product consists of a full-length, mature NEDD8 polypeptide recombinantly expressed in E.coli, conjugated on its c-terminus to AMC. Hydrolysis of the conjugate results in fluorescence observable by excitation at 345nm and emission at 445nm. Typical working range is 50-500nM.
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$245.00
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| Quantity: | 50 μg |
|---|---|
| Molecular Weight: | 8.7 kDa |
| Purity: | >99% by LCMS |
| Readout: | Endpoint/Kinetic |
| Label or Dye: | AMC |
| Detection Method: | Fluorescence |
| Substrate Properties: | Protein-Based Substrate |
| Excitation/Emission (nm): | 345/445 |
| Storage Buffer: | 50mM MES, pH 6.0 |
| Storage | Store at -80˚C. Avoid multiple freeze thaw cycles. |
Certificate of Analysis
Open Certificate in New TabCitations and References
1) Walden H, Podgorski MS, Huang DT, Miller DW, Howard RJ, Minor DL, Holton JM, Schulman BA (2003). “The structure of the APPBP1-UBA3-NEDD8-ATP complex reveals the basis for selective ubiquitin-like protein activation by an E1”. Mol. Cell. 12 (6): 1427–37. PMID 14690597 2) Brown JS, Lukashchuk N, Sczaniecka-Clift M, Britton S, le Sage C, Calsou P, Beli P, Galanty Y, Jackson SP (2015). “Neddylation promotes ubiquitylation and release of Ku from DNA-damage sites”. Cell Rep. 11 (5): 704–14. doi:10.1016/j.celrep.2015.03.058. PMID 25921528.
