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$295.00
Catalog number: SBB-PS0028, 50 μg
This SUMO1 substrate is C-terminally derivatized with a Glycine-Rhodamine-110 (3,6-diamino-9-(2-carboxyphenyl)) fluorophore. The Gly-Rh110 remains quenched until the amide bond between the C-terminal glycine and the Gly-Rh110 compound is hydrolyzed. The efficiency of quenching combined with the powerful signal upon hydrolysis yields a reagent with unparalleled signal-to-background. SUMO1-Rh110 can be used to study the deSUMOylating activity of hydrolases SENP1 and SENP2, or other deSUMOylating enzymes. The substrate activity of SUMO1-Rhodamine110 was determined by measuring the SENP1 catalyzed release of unquenched Gly-Rh110. The Excitation and Emission of this substrate is 485nm and 535nm respectively. This protein was expressed in E.coli.
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$295.00
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| Quantity: | 50 μg |
|---|---|
| Molecular Weight: | 11.5 kDa |
| Purity: | >97% by LCMS |
| Storage Buffer: | 50mM Hepes pH 7.5, 100mM NaCl |
| Storage | Store at −80°C after product arrival. Avoid multiple freeze / thaws. It is recommended to make multiple aliquots after the first thaw. |