Catalog number: SBB-PS0029, 50 μgThis SUMO2 substrate is C-terminally derivatized with a bis-Glycine-Rhodamine-110 fluorophore. The bis-Gly-Rh110 remains quenched until the amide bond between the C-terminal glycine and the rhodamine compound is hydrolyzed. The efficiency of quenching combined with the powerful signal upon hydrolysis yields an unparalleled signal-to-background. SUMO2-Rh110 can be used to study the deSUMOylating activity of hydrolases SENP1 And SENP2, among other deSUMOylating enzymes. The substrate activity of SUMO2-Rhodamine110 was determined by measuring the SENP1 catalyzed release of unquenched Gly-Rh-110. This protein was expressed in E.coli.