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Catalog number: SBB-TR0051-2K, 2000 wells
Mixture of Europium Cryptate Ubiquitin (SBB-TR0014), Cy5-Ubiquitin (SBB-TR0015), and wild-type Ubiquitin (SBB-UP0013) combined in a ratio optimized for TR-FRET based conjugation experiments where long polyubiquitin chains are formed. Conjugation reactions are monitored at Excitation = 304 nm, Emission channel 1 = 620 nm, Emission channel 2 = 665 nm. These proteins were expressed in E.coli.
$3575.00
Catalog number: SBB-TR0051-2K, 2000 wells
Mixture of Europium Cryptate Ubiquitin (SBB-TR0014), Cy5-Ubiquitin (SBB-TR0015), and wild-type Ubiquitin (SBB-UP0013) combined in a ratio optimized for TR-FRET based conjugation experiments where long polyubiquitin chains are formed. Conjugation reactions are monitored at Excitation = 304 nm, Emission channel 1 = 620 nm, Emission channel 2 = 665 nm. These proteins were expressed in E.coli.
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$3575.00
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| Quantity: | 2000 wells |
|---|---|
| Molecular Weight: | 8.5 to 8.9 kDa |
| Purity: | >99% |
| Readout: | Endpoint/Kinetic |
| Label or Dye: | Europium Cryptate & Cy5 |
| Detection Method: | TR-FRET |
| Substrate Properties: | Protein-Based Substrate. Typical experimental concentration is 1x. |
| Excitation/Emission (nm): | 304/620 & 304/665 |
| Storage Buffer: | 50mM Hepes pH 7.5, 100mM NaCl |
| Storage | Store at −80°C after product arrival. Avoid multiple freeze / thaws. It is recommended to make multiple aliquots after the first thaw. |
Certificate of Analysis
Open Certificate in New TabCitations and References
1) Magennis, S. W., Parsons, S., Pikramenou, Z., Corval, A., & Woollins, J. D. (1999). Imidodiphosphinate ligands as antenna units in luminescent lanthanide complexes.Chemical communications, (1), 61-62. 2) Zheng, N., & Shabek, N. (2017). Ubiquitin Ligases: Structure, Function, and Regulation.Annual Review of Biochemistry, (0).
