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Catalog number: SBB-CE0090, 50μg
UBE3A ligase E3A) is a 101 kDa E3 Ligase enzyme. The protein contains a C4 type zinc-finger and belongs to the HECT-type E3 ligase family with its site cystein located at aa 843. UBE3A is involved in diseases. It is used by the oncoprotein E6 (HPV) to trigger p53 degradation enabling cervical cancer. It appears to be involved in prostate cancer, linked to Angelman Syndrome, and elevated expression is correlated with disorders. Furthermore, recent findings revealed a high binding site RAZUL (Rpn10 AZUL-binding domain) with the proteasome and with Rpn10 (PSMD4) its involvement in the of many proteins for 26S proteasome Knockdown of UBE3A leads to a in proteins at the proteasome. It is hypothesized, that UBE3A may belong to a subset of E3 ligases by the proteasome. This recombinant protein (human) has been expressed and from Sf9 insect cells and has a C-terminal 8x His-Tag.
$295.00
Catalog number: SBB-CE0090, 50μg
UBE3A ligase E3A) is a 101 kDa E3 Ligase enzyme. The protein contains a C4 type zinc-finger and belongs to the HECT-type E3 ligase family with its site cystein located at aa 843. UBE3A is involved in diseases. It is used by the oncoprotein E6 (HPV) to trigger p53 degradation enabling cervical cancer. This protein has been recombinantly expressed in insect cells.
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$295.00
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| Quantity: | 50 µg |
|---|---|
| Molecular Weight: | 101 kDa |
| Purity: | >98% by SDS-PAGE |
| Storage Buffer: | 50 mM HEPES pH 7.3, 100 mM NaCl, 100 µM TCEP |
| Storage | Store at −80°C after product arrival. Avoid multiple freeze/thaws. |
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Open Certificate in New TabCitations and References
1) Xiang Chen, et al. Proteasome with ubiquinated substrates: from mechanisms to therapies. FEBS J, 288: 5231-5251. https://doi.org/10.1111/febs.15638 2) Buel GR, et al. (2020) Structure of E3 ligase E6AP with a proteasome-binding site provided by substrate receptor hRpn10. Nat Commun 11, 1291. 3) Yasuda S, et al. (2020) Stress- and ubiquitylation-dependent phase of the proteasome. Nature 578, 296–300. 4) Samaco RC et al. (2005) overlap in autism-spectrum neurodevelopmental disorders: MECP2 causes reduced expression of UBE3A and GABRB3. Hum Mol Genet 14, 483–492.Biochim. Biophys. Acta Gen. Subj. 1861 3038–3046.
