Catalog number: SBB-PS0033, 50 μgUbiquitin-Vinyl Methyl Ester is a potent and specific inhibitor of deubiquitinating enzymes (DUBs) based on a C-terminal electrophilic Vinyl Methyl Ester group. Ub-Vinyl Methyl Ester can be used for activity profiling experiments and DUB inhibitor specificity determination. It targets four of the five major DUB families: UCH, USP, OUT, and MJD, while leaving JAMM metalloproteases uninhibited. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human Ubiquitin- Vinyl Methyl Ester concentration of 1-5 μM.